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| Standard packing | 0.00 Box |
|---|---|
| Supply locations | [DIACETYL FLUORESCEIN] manufacturers, suppliers, exporters in Mumbai, Gandhidham, Kolkata, Varanasi, Visakhapatnam, Chennai, Fujairah, Dubai, Canada BC, Barka, Sohar, Muscat, Oman. Lab chemicals manufacturers, suppliers, exporters in India, UAE Middle East, Barka, Sohar, Muscat, Oman, Canada. [DIACETYL FLUORESCEIN] is available in small packing as well as in bulk. Buy premium quality [DIACETYL FLUORESCEIN] and other lab chemicals from one of the most trusted brands. |
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<em><span style="font-family:georgia,serif;"><span style="font-size:16px;">To prepare a FDA stock solution 5 mg of fluorescein diacetate is dissolved in 1 ml acetone.</span></span></em></div>
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<em><span style="font-family:georgia,serif;"><span style="font-size:16px;">2,5 ml cell culture medium is cooled on ice.</span></span></em></div>
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<em><span style="font-family:georgia,serif;"><span style="font-size:16px;">50 ml of the FDA stock solution is added and this diluted FDA solution is stored on ice.</span></span></em></div>
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<em><span style="font-family:georgia,serif;"><span style="font-size:16px;">Fluorescence-based live-dead assays can be used to evaluate the viability of mammalian cells. ... In this Application Note we present a staining protocol using fluorescein diacetate (FDA) and propidium iodide (PI), which stain viable cells and dead cells, respectively.</span></span></em></p>
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<em><span style="font-family:georgia,serif;"><span style="font-size:16px;">Dilute the fluorescein solution by add- ing enough distilled or deionized water to reach the 500-mL mark on the beaker. Stir. Note: Prepare 1% fluorescein solution by dissolving 1 g of fluorescein in 100-mL distilled water.</span></span></em></p>
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